SACE Biology

SACE Biology practice questions by topic

Original questions for every topic, with worked explanations and feedback on written answers.

Four-option questionsWorked or timed
Written questionsExplanations and data in parts, on a scenario
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Try 4 questions

Question 1
Percentage of each base in four nucleic acid samples
SampleAdenine (%)Thymine (%)Guanine (%)Cytosine (%)Uracil (%)
Sample 1262624240
Sample 2311922280
Sample 3290212525
Sample 4181832320
A laboratory in Adelaide analysed four nucleic acid samples taken from viruses. The table gives the percentage of each base in each sample. Which sample is most likely to be single-stranded DNA?
Question 2
Number of cells in each stage in a sample of 800 cells from a culture
StageNumber of cells
Interphase680
Prophase60
Metaphase30
Anaphase14
Telophase16
Cells in a culture divide every 20 hours, and the cells are at random points in the cycle. The table shows the stage of 800 cells in a sample. About how long does metaphase last?
Question 3
Survival of a small fish species from the southern Coorong after 96 hours at different salinities
Salinity (g L⁻¹)Fish surviving after 96 hours (%)
10100
30100
50100
7095
9060
11010
1300
In a laboratory investigation, groups of 20 fish of a small species collected from the southern Coorong were kept at seven salinities for 96 hours. Based on the table, between which salinities does the upper tolerance limit of this species lie?
Question 4
Temperatures at which half of each DNA sample separated into single strands
DNA sampleSeparation temperature (°C)
Species X with species X86
Species X with species A84.2
Species X with species B79.5
Species X with species C82.1
A survey of native fish in the Coorong compared species X with three other species, A, B and C, by DNA-DNA hybridisation (table). What is the order of relatedness to species X, from the closest to the most distant?

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